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02/14/2009 10:06 PM

Grapefruit Seed Extract Naturally Kill Lyme Cysts?

Canuck
Canuck  
Posts: 1674
Senior Member

I am looking into a zillion things at a time and am putting this study out here as a possible natural method to combat those nasty cysts

It's LONG, as usualSmile

http://www.siv.no/webpro/dokument/ 564000_Citrosept.proof.pdf

Grapefruit Seed Extract is a Powerful In Vitro Agent Against Motile and Cystic Forms of Borrelia burgdorferi Sensu Lato

Lyme borreliosis [1], caused by B. burgdorferi sensu lato, may lead to long-term tissue infection, which may be difficult to cure. The outcome of Lyme borreliosis is highly dependant on the antibiotic treatment [2].

The observation of the ability of B. burgdorferi sensu lato to convert (and reconvert) to cystic forms [3–5] may explain why the infection sometimes is persistent and reactivating.

Therefore, it might be important to eradicate all germative forms (not only the motile form) of the bacterium to obtain a proper treatment for Lyme borreliosis.

Grapefruit-seed extract (GSE) contains bioactive flavenoids (e.g.,hesperitin, resveratrol,and naringenin) and has been shown to possess anti-microbiological effect against bacteria and fungus .

Many studies indicate that GSE is a substance whose therapeutic effect ranks equal to or better than other known anti-bacterial agents.

Positive effects of GSE are decreased levels of TNF-α, Nuclear factor Kb, NO, protection of the gastrointestinal tract against mechanical stress, and has anti-allergic and other antioxidative properties [8, Naringenin, hesperidin and other citrus flavones have been found in plasma and tissue after ingestion [10].

Lactobacillus and bifidobacteria in the gut seems to be insignificantly affected by GSE, and no severe side effects have been observed.

B. burgdorferi sensu lato has a gene for efflux mechanism which may be responsible for antibiotic resistance.

GSE is an efflux inhibitor, which can be used to enhance the activity of antibacterial agents.

For the reasons mentioned above it is reasonable to test the hypothesis that motile and cystic forms of B. burgdorferi sensu lato will be susceptible to GSE, and this is the aim of our study.

The bacterial strain used in our experiments was B. afzelii ACA-1.

Production of mobile spirochetes and

cystic forms was performed according to our previous procedure Grape fruit seed extract 33% (Citrosept; Cintamani Europe AS, 2071 RÃ¥holt, Norway)

was diluted in distilledwater, sterile filtered by a 0.2 μm filter, and diluted geometrically in 5 ml Nalgene tubes from 0.33%–0.00064% in 2 ml of diluted BSK-H medium (dilution 1:100 in distilled water).

The control was diluted BSK-H. Two ml suspension of cystic forms at an age of 1 h was added to each of the tubes giving a final GSE concentration of 0.165%–0.00032%.

Susceptibility testing of mobile spirochetes to GSE was erformed in a final dilution of GSE from 0.165% to0.00032% in BSK-H medim.

Forty micro liter of 107/ml bacteria in logarithmic growth was added, making the final volume 4 ml in each tube.

One control with only BSK-H was used.

To examine if GSE could prevent the conversion of mobile spirochetes to cystic forms, testing was also performed in distilled water for 1 hat 34 °C.

One control with only distilled water was used. Motile bacteria in distilled

water and BSK-H medium were incubated aerobically.

The tubes with the mobile borrelia in BSK-H medium and the cysts in diluted BSK-H were examined by Dark Field Microscopy (DFM) (400×) after 1 h and 7 days to

detect presence of eventual mobile spirochetes and intact cysts.

Bacteria exposed to GSE in water were examined by DFM at 400× to examine the ratio of cyst/bacteria.

Vitalstaining was performed on bacteria exposed to GSE for 1 week by mixing 10 μl of Live/dead BacLightÔ bacterialviability kit (Molecular Probes L-13152 Eugene, OR, USA) with 10 μl of the culture.

This mixture was placed on a glass slide protected with a coverslip.

The BacLight-stained bacteria were examined by UV-microscopy (800×).

The following cultures of spirochetes and GSE were examined by transmission electron microscopy (TEM) as earlier described [13]:

– motile spirochetes incubated for 1 week with GSE at a dilution of 0.0052%, 0.0026%, 0.0013% and a control without GSE in BSK-H medium,

– motile spirochetes incubated for 1 h with GSE at a dilution of 0.165%, 0.0825%, 0.0413%, 0.01% and a control

without GSE in BSK-H medium,

– motile spirochetes incubated for 1 h with GSE at a dilution at 0.0413%, 0.0052%, 0.0013%, 0.00064% and

a control without GSE in distilled water, and

– 1 h old cysts incubated for 1 h with GSE at a dilution of 0.021%, 0.01%, 0.0052%, 0.0013%, 0.00064% and a control without GSE.

GSE-exposed cultures were

recultivated in BSK-H medium as earlier described [13] to confirm or invalidate the existence of viable bacteria.

MBC of the mobile spirochetes was determined by recultivation of GSE-exposed

spirochetes, and the lowest GSE concentration where no growth occurred was set as the MBC value.

The MIC value for mobile spirochetes was determined according to the lowest GSE concentration, which gave reduced multiplication when examined in DFM.

When the susceptibility testing for

mobile spirochetes was performed in distilled water, the rate of conversion was strongly dependent on the GSE concentration.

After incubation for 1 h at 34 °C the

number of spirochetes converted to cysts

ranged from none at GSE concentration of

0.165%–0.0052%, 10% at 0.0028%, 20%

at 0.0013%, 95% at 0.00064%, and > 95%

in the control when examined in DFM.

By TEM, the dilution of 0.0013% showed a

very few cysts; the dilution of 0.00064%

showed many normal cysts but not as many

as in the control.

Susceptibility testing of normal mobile

borrelia exposed to GSE at 34 °C for

1 h revealed motile bacteria at concentrations ≤ 0.01%.

After 5 weeks of incubation in fresh BSK-H medium, motile spirochetes were observed only at the dilutions ≤ 0.021%.

By TEM some bacteria with normal appearance (compared to the control)

were observed in the concentration

of 0.041%, which is set to be the MBC (Figure 1).

When the mobile spirochetes were exposed to GSE for 1 week at 34 °C in fresh BSK-H medium the estimated MBC was 0.0052% and MIC was ≤ 0.00032%.

Four weeks of cultivation revealed 107 bacteria/ml in the 0.0026% dilution.

However, BacLightÔ showed green structures (green color indicates living organisms) only from the 0.0013% dilution.

This corresponded well with results obtained by TEM.

Rupturing was observed by TEM and DFM for 100% of the 1 h old cysts which had been incubated in GSE from Figure 1.A.

Spirochetes incubated for 1 h at 34 °C with 0.165% GSE diluted in BSK-H medium.

Only a very few pycnotic bacteria were present. Most bacteria were completely dissolved.B.

Spirochetes exposed to 0.041% GSE. The bacteria have a normal ultrastructure.

TEM. Bar = 500 nm. A.

One hour old cysts incubated for 1 h at 34 °C in BSK-H medium with 0.0013% GSE. A few normal and some dissolved cysts were present. B.

The same cysts as in A, but exposed to 0.00064% GSE. The number of normal cysts present was approximately the same as in the control. C.

The cysts in B were transferred into fresh BSK-H medium and incubated for 5 weeks in 34 °C.

Many normal spirochetes were present. TEM. Bar = 1,000 nm.Ø. Brorson, S.-H.

Brorson Citrosept Against Borrelia burgdorferi Infection 35 · 2007 · No. 3 © URBAN & VOGEL 3 0.165%–0.021%; for GSE-dilutions from 0.01%–0.00064%

rupturing was observed for 90%–5% (most rupturing for the less diluted GSE).

For the negative control > 98%

cysts occurred intact.

When transferred to BSK-H medium motile bacteria were observed after following incubation time:

14 days for the control and GSE dilution 0.00032%;

5 weeks for the 0.00064% dilution;

no re-growth for higher concentrations (Figure 2).

Therefore, the MBC was calculatedto 0.0013%.

The highest GSE concentrations made the bacteria and cysts disappear completely, leaving only small uncharacteristic fragments; at lower GSE-levels the membranes showed herniation and disruption, and the contents had leaked out.

The MBC was strongly dependent on the length of the incubation.

GSE was very active even for very short incubation times, in agreement with previous results.

The MBC obtained by DFM for the motile bacteria agreed well with the TEM results.

Presence of GSE reduced the conversion from spirochetes to cysts when the susceptibility testing was performed in distilled water.

This study was performed in vitro and further studies are needed to demonstrate eventual effects in vivo.

From our results it will be rational to test the hypothesis that a combination of GSE and antibiotics will be efficient in the treatment of resistant Lyme borreliosis.

Post edited by: Canuck, at: 02/14/2009 22:14

used quotes to enlarge text & broke up longer paragraphs since they were so technical. bettyg, iowa leader Smile

Post edited by: Bettyg, at: 10/15/2010 12:36 AM

Reply

03/24/2009 04:17 AM
mem6757

I am going for the GSE as a natural Cyst Buster,If i am symptom free a few months from now i will guess that it worked.I am not willing to go off Samento or Carnivora to test though ..too scary as it could manifest itself slowly again and have to go through the whole deal again Smile Just a short copy below Smile

Pretty Sure its from your same site Tina Smile

The highest GSE concentrations made the bacteria and

cysts disappear completely, leaving only small uncharac-

teristic fragments; at lower GSE-levels the membranes

showed herniation and disruption, and the contents had

leaked out. The MBC was strongly dependent on the length

of the incubation. GSE was very active even for very short

incubation times, in agreement with previous results [7].

The MBC obtained by DFM for the motile bacteria agreed

well with the TEM results. Presence of GSE reduced the

conversion from spirochetes to cysts when the susceptibil-

ity testing was performed in distilled water. This study was

performed in vitro and further studies are needed to dem-

onstrate eventual effects in vivo. From our results it will be

rational to test the hypothesis that a combination of GSE

and antibiotics will be efficient in the treatment of resistant

Lyme borreliosis


03/24/2009 04:39 AM
mem6757

A Must Read ..Samento Is a Cyst Buster.. Great Information for all!

http://www.healingwell.com/community/default.aspx?f=30& m=1404526


03/24/2009 10:06 AM
adamswife

Ok - limited on time but the word "cyst" caught my eye..before I read all this and let it in my limited brain space...are you guys talking about skin cysts? like zit-cysts but different (worse?) if so I am going to FREAK out.

03/24/2009 10:33 AM
mem6757

adams...No not at all..talking the cyst form of Lyme spirochetes..with a protective coating.. microscopic Smile

03/24/2009 11:00 AM
adamswife

oh ok good Wink

10/14/2010 09:35 AM
Supermom2
 
Posts: 269
Member

BUMPING THIS

There have been several threads lately concerning which herbs and supplements are useful in Lyme treatment.

It was new information for me, so there may be others that would benefit from seeing it.

supermom2

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