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01/27/2011 12:08 AM

BIOFILMS OF BB & CHRONIC BORRELIOSIS

Bettyg
 
Posts: 32210
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74 pages of this study with 60-70 being GRAPHICS galore!

http://www.molecularalzheimer.org/files/ Biofilm_New_Haven_ppt_Read-Only_.pdf

skim thru the wonderfully detailed graphics here; i saw 1 alzheimer's graph and muchmore!

bettyg, iowa leader

Post edited by: Bettyg, at: 01/27/2011 12:09 AM

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01/30/2011 01:49 PM
wlkthlne
wlkthlne  
Posts: 1844
Senior Member

Hope I did this right, Betty wanted me to add this from basic discussions...

There was a topic on biofilm some weeks ago..Think "Inky" might have bought it up????

Anyhow, I came across this...and thought I would share.

She is a little hard to understand...but as you listen it gets easier...

Cought somethings I have not heard before... here are two links...

if the first link does not get you there...try the second one..

http://neuro-lyme.com/videoblog/dr-eva-sapi-bacterial- biofilms-and-lyme-disease-2/

http://www.youtube.com/watch?v=AmvgOfIN_8c& feature=player_embedded

hope today is a better day for everyone..

wlkthlneUnsure

Post edited by: Bettyg, at: 12/20/2011 01:20 AM


12/19/2011 01:25 AM
Bettyg
 
Posts: 32210
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Biofilms: Gated Communities

http://www.treatlyme.net/articles/2011/12/2/biofilms-gated- communities.html

Friday, December 2, 2011 at 6:02AM

Marty Ross MD

Post a Comment ..none yet

Biofilms are protective communities where various forms of the Lyme bacteria can hide from prescriptive antibiotics, herbal antimicrobials, and the immune system.

In chronic Lyme and associated diseases they can cause:

■treatment resistance and

■relapse once antibiotics or herbal antimicrobials are stopped.

Biofilms are not unique to borrelia, the Lyme disease bacteria.

They are known to occur based on scientific studies in various infections such as staph infections of the skin.

In Lyme disease, Alan MacDonald MD, has shown biofilms to exist based on his microscopic exams of brain tissues.

Composition

In biofilms bacteria produce and cover themselves in a layer of slime composed of mucopolysaccharides.

To add structure, the bacteria recruit a protein found in blood called fibrinogen which they then convert to the protein fibrin.

Because the fibrin gives structure, the bacteria in biofilms can rid themselves of their outer protein coverings making it harder for the immune system to react against them.

In addition the slime layer covers the germs so the immune system cannot see them and antibiotics or antimicrobial supplements cannot reach the germs.

Within the biofilms, the germs establish highly organized structures and functions where they communicate using various chemical messengers, excrete waste through channels and perform other complex activities to promote the longevity of the community.

Biofilms require calcium and magnesium and contain other minerals and heavy metals.

Biofims and Treatment

In my practice I intentionally treat biofilms

■at the end of treatment to prevent relapse or

■when a treatment is not progressing well due to treatment resistance.

Some physicians address biofilms at the very beginning or throughout the entire course of a treatment.

In my practice I do not do this because my observation is that 90% or more of my patients get well without specific biofilm treatments.

Even though we know based on the work of Alan MacDonald MD that biofilms exist in brain tissues of people with Lyme, there is no scientific evidence that they occur in every individual who has Lyme and associated diseases.

Furthermore, even if they do exist in all that have borrelia infection, there is no scientific evidence that they universally block treatments.

How to Treat Biofilms

Based on my review of biofilm treatments there are two scientifically supported approaches to eliminate biofims in Lyme Disease and many theoretical treatment approaches.

Research-based Treatments

Eva Sapi PhD and her colleagues are performing groundbreaking Lyme Disease research. In the past 2 years she has published two articles based on petri dish experiments in her lab.

The first published over a year ago shows that the herbs Banderol and Samento used together completely eliminate biofilm communities and eradicate nearly every germ living in them.

Her more recent study of 5 different antibiotics shows that prescription tinidazole decreases the size of biofilm communities and eliminates the germs living in them by over 90 percent.

Based on my review of her research, I believe the best antimicrobial approach that eliminates biofilms and the germs that live in them are the herbs Banderol and Samento.

Prescriptive tinidazole is a very close second though.

Theoretical Treatments

As I noted above biofilms require magnesium and calcium, have various minerals and heavy metals, and contain fibrin protien structures and mucopolysaccharides substances.

Theoretical treatments address these various components.

Some physicians treat biofilms by

■starving the germs by eliminating or limiting calcium and magnesium,

■removing minerals and heavy metals with the chelating agent EDTA, and

■breaking up the protien fibrin matrix with enzymes like Lumbrokinase or nattokinase.

In theory, I understand these approaches. However, I think such approaches are overkill.

Now I admit that I am a minimalist when it comes to treating Lyme and associated diseases.

I believe that physicians should treat using as few supplements or prescriptions as possible to gain the maximum benefit.

Even though I work with supplements for instance, I do not think it is useful or appropriate for most patients to leave a medical office or a store with bags full of supplements.

Regarding eliminating calcium and magnesium or limiting them, I do not support this.

Biofilms exist in us, so of course they require the same minerals that we do to survive.

I do not think we should starve the host (a person with Lyme) to kill the germ.

The only theoretical approach I use in my practice is the supplement Lumbrokinase. It breaks down the fibrin protien skeleton that holds the biofilm together.

This alone is enough to break up the biofims. Using Lumbrokinase alone I have seen great improvements in my patients with treatment resistant Lyme and decreased episodes of relapse.

An alternative to the Lumbrokinase is nattokinase, but it is a much weaker fibrin dissolving enzyme and I do not find it effective.

Biofilm Treatment Approach

Treat biofilms in the last four months of treatment to prevent relapse or anytime in treatment when resistance to antibiotics or herbal antimicrobials is blocking progress.

So how do I design a treatment for biofilms? My favorite and most effective approach is to use Banderol and Samento.

When using these two herbs, I stop all prescritpive antibiotics.

Dr. Sapi's work suggest this is the most effective approach, and this is what I observe in my practice as well.

Another approach is to use prescritipive tinidazole in an antibiotic approach.

I use Lumbrokinase, if an antibiotic approach is working fairly well, or if Banderol and Samento do not work, or if a person cannot tolerate the nausea or abdominal cramping that can occur with tinidazole.

It is safe to add Lumbrokinase to any antibiotic regimen.

To read how to use Banderol and Samento or Lumbrokinase or to purchase these supplements click on the Links in this section.

One word of caution, when treating biofilms, sometimes die-off Herxheimer reactions can occur. In a die off reaction the immune system makes more inflammatory chemicals that can temporarily make the Lyme and associated diseases symptoms worse.

To read about these reactions and the treatment approach I use for them see the Treatment Manual:

Supplements and Advanced Treatments in Lyme and Associated Diseases.

Final Word

As Dr Brooke and I note on this site, often supplements offer better approaches to treat this illness than prescription-only treatment approaches.

The examples laid out above regarding treating biofilms with Banderol and Samento or Lumbrokinase provide perfect examples of our claim.

Read additional articles or supplement blog entries on this site to see other examples of how you can improve your prescription based approach.

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12/20/2011 01:33 AM
Bettyg
 
Posts: 32210
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Biofilm-Forming Bacteria

http://flash.lymenet.org/scripts/ultimatebb.cgi? ubb=get_topic;f=1;t=113475;p=0

Al

LymeNet Contributor

Member # 9420

posted 12-17-2011 12:45 AM

Interfering With The Ability Of Biofilm-Forming Bacteria To Sense Starvation Increases Their Susceptibility To Antibiotics

21 Nov 2011

10-15 minute VIDEO here too

Many infections, even those caused by antibiotic-sensitive bacteria, resist treatment. This paradox has vexed physicians for decades, and makes some infections impossible to cure.

A key cause of this resistance is that bacteria become starved for nutrients during infection. Starved bacteria resist killing by nearly every type of antibiotic, even ones they have never been exposed to before.

What produces starvation-induced antibiotic resistance, and how can it be overcome?

In a paper appearing in Science, researchers report some surprising answers.

"Bacteria become starved when they exhaust nutrient supplies in the body, or if they live clustered together in groups know as biofilms," said the lead author of the paper, Dr. Dao Nguyen, an assistant professor of medicine at McGill University.

Biofilms are clusters of bacteria encased in a slimy coating, and can be found both in the natural environment as well as in human tissues where they cause disease. For example, biofilm bacteria grow in the scabs of chronic wounds, and the lungs of patients with cystic fibrosis. Bacteria in biofilms tolerate high levels of antibiotics without being killed.

"A chief cause of the resistance of biofilms is that bacteria on the outside of the clusters have the first shot at the nutrients that diffuse in," said Dr. Pradeep Singh, associate professor of medicine and microbiology at the University of Washington in Seattle, the senior author of the study.

"This produces starvation of the bacteria inside clusters, and severe resistance to killing."

Starvation was previously thought to produce resistance because most antibiotics target cellular functions needed for growth. When starved cells stop growing, these targets are no longer active. This effect could reduce the effectiveness of many drugs.

"While this idea is appealing, it presents a major dilemma," Nguyen noted. "Sensitizing starved bacteria to antibiotics could require stimulating their growth, and this could be dangerous during human infections."

Nguyen and Singh explored an alternative mechanism.

Microbiologists have long known that when bacteria sense that their nutrient supply is running low, they issue a chemical alarm signal. The alarm tells the bacteria to adjust their metabolism to prepare for starvation. Could this alarm also turn on functions that produce antibiotic resistance?

To test this idea, the team engineered bacteria in which the starvation alarm was inactivated, and then measured antibiotic resistance in experimental conditions in which bacteria were starved.

To their amazement, bacteria unable to sense starvation were thousands of times more sensitive to killing than those that could, even though starvation arrested growth and the activity of antibiotic targets.

"That experiment was a turning point," Singh said. "It told us that the resistance of starved bacteria was an active response that could be blocked. It also indicated that starvation-induced protection only occurred if bacteria were aware that nutrients were running low."

With the exciting result in hand, the researchers turned to two key questions.

First does the starvation alarm produce resistance during actual infections?

To test this the team examined naturally starved bacteria, biofilms, isolates taken from patients, and bacterial infections in mice. Sure enough, in all cases the bacteria unable to sense starvation were far easier to kill.

The second question was about the mechanism of the effect. How does starvation sensing produce such profound antibiotic resistance?

Again, the results were surprising.

Instead of well-described resistance mechanisms, like pumps that expel antibiotics from bacterial cells, the researchers found that the bacteria's protective mechanism defended them against toxic forms of oxygen, called radicals.

This mechanism jives with new findings showing that antibiotics kill by generating these toxic radicals.

The findings suggest new approaches to improve treatment for a wide range of infections.

"Discovering new antibiotics has been challenging," Nguyen said.

"One way to improve infection treatment is to make the drugs we already have work better. Our experiments suggest that antibiotic efficacy could be increased by disrupting key bacterial functions that have no obvious connection to antibiotic activity."

The work also highlights the critical advantage of being able to sense environmental conditions, even for single-celled organisms like bacteria. Cells unaware of their starvation were not protected, even though they ran out of nutrients and stopped growth.

This proves again that, even for bacteria, "what you don't know can hurt you."

Posts: 674 | From North East | Registered: Jun 2006

**********************

MattH

LymeNet Contributor

Member # 30846

posted 12-17-2011 11:29 AM

So does this track with Dr K talking about the idea of using Hyaluronic Acid (HA)as bait for the ketes?

As I interpreted what he said, you take the ABX or what ever you are using to kill the bacteria and then take the HA to attract ketes to the gut. They are then killed by the ABX that has been recently ingested.

So does that mean we should all be eating a lot more sugar??? Just in time for the holidays!!!

All the Best, MattH

*************************

Catgirl

LymeNet Contributor

Member # 31149

posted 12-18-2011 12:34 PM

Wow, fascinating stuff, Al! So if we take lots of nutrients the bacteria won't turn on their alarm, and eventually die from the abx. Too cool.

I need to take a lot more vitamins and nutrients. I've been too lax on this.

Different subject, but did anyone catch Horrowitz's speech (Ilads conference)? [u]He said that glutathione opens up the pathways. That video is worth the download ($15).

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Post edited by: Bettyg, at: 12/21/2011 12:44 AM


12/20/2011 04:00 PM
mem6583

Lots of good stuff, love this thread!

01/10/2014 12:25 AM
Bettyg
 
Posts: 32210
VIP Member
I'm an Advocate

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